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In this work, we explored the all-natural variation in tolerance to Al3+ between high and reasonable tolerant Brachiaria species and characterized their transcriptional variations during anxiety. We identified three QTLs (quantitative trait loci) involving root vigour during Al3+ anxiety inside their hybrid progeny. By integrating these results with a new Brachiaria research genome, we identified 30 genes putatively responsible for Al3+ tolerance in Brachiaria. We observ