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We assessed the relationship between nighttime ecological noise and semen high quality of males who visited for virility assessment. This is a retrospective cohort research of 1,972 male patient that has done semen analysis between 2016-2018 at just one virility center of Seoul, Southern Korea. We utilized ecological sound data of National Noise Suggestions System (NNIS), Korea. Using semiannual nighttime noise measurement closest into the period of semen sampling, individual sound exposures at