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Fatigue is an important factor affecting modern flight safety. It can easily lead to a decline in pilots' operational ability, misjudgments, and flight illusions. Moreover, it can even trigger serious flight accidents. In this paper, a wearable wireless physiological device was used to obtain pilots' electrocardiogram (ECG) data in a simulated flight experiment, and 1440 effective samples were determined. The Friedman test was adopted to select the characteristic indexes that reflect the fatigue state of the pilot from the time domain, f