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Background Serum biochemistry and ultrasonography can be useful diagnostic tools in evaluating the general health condition of long-tailed macaques in rescue and rehabilitation centers. Methods This study was conducted to determine and correlate the serum biochemistry profile of 24 apparently healthy male and female rescued long-tailed macaques (LTM) with the body weight, crown-rump length, and ultrasonic histogram of liver parenchyma, gallbladder lumen, and renal cortices. Results There were no sex-related differences in serum biochemi