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Fecal samples were collected at baseline and 3-month post-randomization for 16S rRNA-based microbiome analysis. Within the CSM intervention group (n = 3, Shannon diversity, richness, and beta diversity measures at baseline did not predict benefit from the CSM intervention at 3 months, as measured by change in abdominal pain and quality of life. Based on both alpha and beta diversity, the change from baseline to follow-up microbiome bacterial taxa did not differ between CSM (n = 25) and usual care (n = 25). Baseline microbiome does not
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