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A total of 408 samples obtained from 136 ornamental fish from 36 species were tested. Using the culture method was isolated from 69 fish (50.1%) and 99 samples (24.3%). Sequence analysis of gene fragments coding for the Hsp65 protein of 99 isolates revealed occurrence of 13 species of mycobacteria , and . The obtained results indicate a significant role of ornamental fish as a source of mycobacteria which are potentially dangerous,especially to humans. The obtained results indicate a significant role of ornamental fish as a source of mycobacteria which are