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We created and applied an alternate sampling method based on molecular analysis of water samples for parasite DNA. We sequenced the small subunit ribosomal DNA (ssrDNA) of Ich isolates collected through the Klamath River, then created and validated a novel qPCR assay (SYTO9) that targets Ich ssrDNA. Our assay has actually much better specificity than previously posted assays, with strong linearity, efficiency and re
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