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A high correlation of MICs and zone diameters was observed for all Enterobacterales (|r| 0.8, P 0.001) and a relatively high correlation was found for P. aeruginosa (|r| = 0.71, P 0.001). The zone diameter ECOFF was 24 mm for E. cloacae, E. coli and K. pneumoniae, 26 mm for P. mirabilis and 21 mm for P. aeruginosa. We determined MIC and zone diameter ECOFFs for cefoselis against four Enterobacterales species and P. aeruginosa. The establishment of ECOFFs for cefoselis provides clinicians with helpful guidance to different