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Inadequate placental perfusion is closely associated with a few pregnancy-associated diseases. Galectin-9 (Gal-9) has numerous regulating functions in innate and transformative resistance during infection, tumor growth, and organ transplantation. We applied immortalized human first-trimester extravillous trophoblast cells (HTR8/SVneo) for our functional research and examined the effects of Gal-9 on apoptosis, cytokine production and angiogenesis of HTR8/SVneo cells. Gal-9 inhibited th