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As a bad form of early-life stress (ELS), maternal separation (MS) can affect the introduction of cognition and behaviors of teenage rats. Brain-derived neurotrophic factor (BDNF) is active in the legislation of mind development and purpose, but the molecular systems through which BDNF regulates brain purpose and behavior in MS with different stressor strengths stay uncertain. This descriptive study characterized the levels of BDNF in the prefrontal cortex (PFC) and plasma