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ELISpot was also performed to measure HER2-specific T cells in splenocytes. In addition, infiltration of CD3 T cells in brain metastatic nodules was analyzed with immunohistochemistry. Flow cytometry detected increased number of activated dendritic cells in lymph nodes in mice treated with cancer vaccine. ELISpot revealed abundant IFN-γ-expressing T cells in the spleen. Mice treated with abdominal flap-embedded cancer vaccine extended median survival by 9 days over the control group (p0.05). Abdominal flap-embedded cancer vaccine eff