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Previous clinical observational studies have suggested that orthodontic tooth movement (OTM) is related, at least partly, to the mass and/or capabilities of the masticatory muscles. Our study aimed to examine the influence of masticatory muscle mass on the OTM in an animal experimental model in which the masseter muscle was modulated by botulinum neurotoxin type A (BTX) injection. Eighteen Wistar rats were equally divided into two groups BTX injection and control. BTX was injected bilaterally into the masseter muscles. Three days after t