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Metabolome evaluation and proteomics experiments confirmed the recommended biosynthetic pathway in Thermomicrobium roseum These results clarify the biosynthetic route-function interactions within the NTR superfamily of enzymes. Appreciably, these outcomes offer an alternate route to produce F420 in Gram-negative design organisms and unveil just one more biochemical element of this path to be investigated by artificial microbiologists. Copyright © 2020 American Society for Microbiology.The pe