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Outcomes showed that NOR really inhibited the methane production with an 50% inhibitory focus (IC5 of 0.41 mM. In inclusion, with expanding of exposure time, inhibitory impact progressively strengthened as well as the IC50 values decreased to 0.16 mM and 0.07 mM in the second and third feeding cycle, respectively. Nevertheless, when the inhibitor in supernatant was removed, the performance restored and the relative methane yield increased by 9 times from 25.38 mL/g VS to 257.05 mL/g VS. The change of