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In this research, we identified 209 PagERF transcription elements by examining the P. alba × P. glandulosa genome. We analyzed their amino acid sequences, molecular weight, theoretical pI (Isoelectric point), uncertainty index, aliphatic index, grand average of hydropathicity, and subcellular localization. Most PagERFs had been predicted to localize within the nucleus, with just a few PagERFs localized in the cytoplasm and nucleus. Phylogenetic evaluation split the PagERF proteins into t