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Objective To analyze the characteristics of hospital nursing professionals with the presence of stress, and to associate this with capillary cortisol. Method A cross-sectional, exploratory and correlational study, conducted in a hospital in São Paulo, Brazil. A total of 164 nursing professionals participated; the Perceived Stress Scale was administered, and hair samples were obtained for laboratory analysis. Data were entered into a Microsoft Excel spreadsheet (201, and then into Microsoft Office and the R software, version 3.2.2. Res