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Among these tissues, spinal cord lysates were found to have PFN1 bands at higher molecular weights recognized with anti-PFN1. Moreover, the pull-down assay using His -PFN1 showed that Myelin Binding Protein (MBP) was present in the insoluble fraction. Our analysis of PFN1 aggregation in vivo revealed further details of mutant PFN1 aggregation and its possible complex formation with other proteins, providing new insights into the ALS mechanism. Our analysis of PFN1 aggregation in vivo revealed further details of mutan