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japonicus. This was because that higher salinity levels increased the uptake rate constant and bioconcentration factor of 4-MBC and also further exacerbated the oxidative stress induced by exposure to 4-MBC in T. japonicus. Our study demonstrated that understanding how salinity affects the toxicity of 4-MBC is important for accurate assessment of the risk of 4-MBC in the aquatic environments.Ecological risks of a pollutant are typically assessed via short-term exposure of model organisms to that single compound. Such tests are informat