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Moreover, Ag+ catalyzed chalcopyrite corrosion and accelerated biofilm formation by producing a loose porous Ag2S layer and Ag0 to decrease the resistivity. The live/dead ratio was small with a range of 0.31-1.38, suggesting that dead cells were a great slice during the whole life-cycle of biofilm on chalcopyrite. This report offers a profound insight into the promotion mechanism of Ag+ on adhesion behaviors and biofilm formation by thermoacidophilic archaeon under extremely acidic conditions.This study investigated the applicability of