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Additionally, we identified the exclusive presence associated with the espACD operon in M. kansasii subtype I, and then we verified its part into the pathogenicity of M. kansasii in a cell illness design. The espACD operon is solely present in mycobacterial species that creates phagosomal rupture in host phagocytes and it is considered an important determinant of ESX1-mediated virulence in pathogenic mycobacteria. Comparative transcriptome analysis ass