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Values of dehydrogenase activity were lower if compared with the mechanically and biologically treated compost, which indicates lower compost maturity. The biomass production of Brassica napus L. and Fetuca rubra L. was higher in the variant with the application of green compost. The influence on Hordeum vulgare L., Cannabis sativa L., and Sinapis alba L. depended on the plant type and the compost used. Nevertheless, the compost from green waste was less toxic. The evidence from this study suggests that the mechanical-biological treatment