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Florfenicol is widely used to control diseases in aquatic animals, and is used extensively to treat streptococcosis-caused by Streptococcus agalactiae-in the commercially important fish tilapia. There are known issues with the development of florfenicol resistance in Streptococcus agalactiae, but the underlying resistance mechanisms are not clear, a situation currently preventing optimal deployment of antibiotics. Here, we examined the induction of resistance by successively increasing the concentrations of florfenicol, and then used RNA
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