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Another key finding was the activation of cytoplasmic streaming in abscission-zone cells detected by electron microscopy. Our results provide a novel insight into the molecular basis of fruit abscission physiology in response to cold stress in apple.Much evidence suggests that Amazonia and the Atlantic Forest were connected through at least three dispersion routes in the past the Eastern route, the central route, and the Western route. However, few studies have assessed the use of these routes based on multiple species. Here we present