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Hindlimb muscle mass and also the heart were excised 24 h post reperfusion. RIPC ahead of I/R upregulated autophagy in the rat heart at 24 h post reperfusion. In vitro, autophagy inhibition or stimulation just before RIPC, correspondingly, either ameliorated or stimulated the cardioprotective result, calculated as improved cell viability to mimic the preconditioning effect. Recombinant interleukin-6 (IL-6) therapy just before I/R