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Electron-withdrawing sulfonyl group and bromine substitution significantly decreased amount and kinetics of NER formation, respectively. This study provides the first direct evidence for the formation of type I NERs in humic substances and implies a "Trojan horse" effect of such NERs in the environment.Veterinary antibiotics are widely used in many countries worldwide to treat diseases and protect the health of animals. However, the effects of sulfonamide antibiotics introduced via manure and wastewater irrigation on nitrogen (N) loss in the soil-plant syst