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A comprehensive study of the toxicity of lanthanides (LN) in relation to the media composition will enhance the prediction of their potential adverse effects for living organisms. Here we examined the effect of different media on the V. faba root elongation and on the cytotoxic (mitotic index) and the genotoxic (micronucleated cell number) effects from toxicity tests with Ce, Gd and Lu (100, 800 and 6400 μg L-1). Three different exposure media were selected the standard Hoagland media (SH); an alternative SH, without phosphates (SH-P); and