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To investigate Krüppel-like factor 17 ( ) expression in normal and gastric cancer tissues and cell lines. Levels of KLF17 mRNA and protein in GES-1 normal gastric mucosal cells, and NCI-N87, SGC-7901, BGC-823 and HGC-27 gastric cancer cells were analysed by quantitative polymerase chain reaction (qPCR) and western blot. Differences in expression between gastric cancer and adjacent tissues were analysed by qPCR and immunohistochemistry. Invasion/migration effects of overexpression in BGC-823 and HGC-27 cells were analysed by wound-healing