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Dicyandiamide (DCD) is commonly used as nitrification inhibitors which has the potential to reduce nitrogen loss from paddy soils. In paddy systems, periphytic biofilms are commonly presented at the soil/water interface and show significant effects on nutrient cycling. However, the interaction between DCD and periphytic biofilms in paddy and subsequent effects on nitrogen cycling is unclear. In this work, microcosm experiments were carried out to study the interaction between the periphytic biofilms and DCD and the potential influence on n