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The aim of this study was to investigate the mechanism of hydrogen gas on hind limb IR injury. Male C57BL/6 mice were randomly divided into three groups sham group (Sham), ischemia-reperfusion group (IR), IR plus H inhalation group (IR+H ). IR was induced by interrupting hind limb blood flow for 3h, followed by 4h of reperfusion, and H was administered by inhalation throughout the reperfusion process. Our data show that H inhalation could significantly decrease the infarct-affected tissue volume (P 0.05), attenuate the degree of mo
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