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The outcomes of this study expand scientific understanding on the genes and pathways involved in EPS biosynthesis by marine bacteria.Nocardioides aquaticus KCTC 9944T is an aerobic, non-motile, Gram-positive, psychrotolerant, non-spore-forming bacterium isolated from the surface water of Ekho Lake in the Vestfold Hills, East Antarctica. This meromictic lake separated from Antarctic seawater thousands of years ago exhibits steep gradients of salinity and temperature in the upper layer of the water column. The cells of N. aquaticus thriv