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EREG promoter hypermethylation in HPV cells was confirmed using methylation-specific PCR (MS-PCR). We conclude that EREG is the target of epigenetic regulation in HPV HNCs and its suppressed expression through promoter hypermethylation is associated with the development of HPV-associated HNCs. We conclude that EREG is the target of epigenetic regulation in HPV+ HNCs and its suppressed expression through promoter hypermethylation is associated with the development of HPV-associated HNCs.Toxoplasmosis is a zoonotic disease and a global fo