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We also show that pharmacological inhibition of lactate dehydrogenase is able to reduce glucose-induced shifts towards aerobic glycolysis. This method is timely given the recent advances in our understanding of new respiratory epithelial subtypes that can only be generated in vitro through culture at ALI and will open new avenues to measure real-time metabolic changes in healthy and diseased respiratory epithelium, and in turn the potential for the development of novel therapeutics targeting metabolic-driven disease phenotypes.Both skin