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SignificanceThe magnitude of the CO2 fertilization effect on terrestrial photosynthesis is uncertain because it is not directly observed and is subject to confounding effects of climatic variability. We apply three well-established eco-evolutionary optimality theories of gas exchange and photosynthesis, constraining the main processes of CO2 fertilization using measurable variables. Using this framework, we provide robust observationally inferred evidence that a strong CO2 fertilization effect is detectable in globally distributed eddy co