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The present study provided more genomic data to know the diversity of the mitochondrial genome and its molecular evolution in Schizothoracinae.Accumulating evidence has demonstrated that microRNAs (miRNAs or miRs) play an important role in the diagnosis and prognosis of tumors. In the case of cholangiocarcinoma (CCA), miRNAs may serve as potential tumor biomarkers and therapeutic targets. Based on The Cancer Genome Atlas (TCGA) database, fold change 2 was used to screen out miRNAs with differential expression in patients with CCA. U