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Our study hence shows that the precise method used needs to be considered when ANI is used to delineate prokaryotic species. We also suggest that one could initially have to figure out a suitable cut-off price for a specific taxon set, based on the intraspecific diversity of this group, before conclusions on conspecificity of isolates could be made, and that the ensuing species hypotheses be confirmed with analyses considering evolutionary record within the polyphasic way of taxonomy.Intr