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Blood species identification of human and animals has attracted much attention in the areas of customs inspection and forensic science. The combination of vibrational spectroscopy and machine learning has been proven to be feasible and effective for this purpose. However, the popularization of this technology needs instrument which is compact, robust and more suitable for field application. Besides the quantity of the blood sample should be as little as possible. In this study, we proposed a system using echelle Raman spectrometer combined with surf