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Click chemistry is not a single specific reaction, but describes ways of generating products following examples in nature. Click reactions occur in one pot, are not disturbed by water, generate minimal and inoffensive byproducts, and are characterized by a high thermodynamic driving force that drives the reaction quickly and irreversibly to high yield of a single reaction product. Therefore, since over 15 years it has become a very useful bio-orthogonal method to prepare chemical cross-linked biopolymer-based hydrogel in presence of e.g