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These O-glycans were suc-cessfully printed onto epoxy glass slides as an O-glycome shotgun microarray. We used this novel array to explore binding activity of serum IgM in healthy person and NSCLC patients at different cancer stages. This novel strategy provides access to complex O-glycans in signifi-cant quantities and may offer a new route to discovery of potential diagnostic disease biomarkers.Phosphotyrosine (pTyr) signaling complexes are important resources of biomarkers and drug targets which often need to be profiled with enough th