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suggesting a more limited role for bacterial methionine metabolism genes in host lifespan effects. In a parallel set of experiments we created a distinct bacterial strain that expressed lifespan-extending methionine metabolism genes and show that this strain can extend fly lifespan. Therefore, this work identifies specific bacterial genes that influence host lifespan, including in ways that are consistent with the expectations of methionine restriction. Copyright © 2020 American Society for Microbiology.Enterotoxigenic Escherichia coli