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We showed that the morphology of ESC-derived aggregates and Zscan4 phrase are strictly intertwined. Our information suggest that ESC Zscan4 metastate may be marketed if the adhesive surface conditions guide cellular self-aggregation into 3D dome-like structure, by which both cell-material interactions and cell-cell contact tend to be supporting for Zscan4 expression. Copyright © 2020 Natale, Angrisano, Pistelli, Falco, Calabrò, Netti and Ventre.Micr