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Co-liquefaction was combined with hydrothermal liquefaction (HTL) aqueous phase (AP) recirculation to improve the practicality of HTL process. The Chlorella powder (CL), soybean straw (SS), and their mixture (CS) with ratio 11 were processed at 300 °C for 20 min, and the AP was recirculated four times. The yield of CS bio-crude was increased (from 24.28% to 31.83%) by co-liquefaction, but remained stable during AP recirculation. By contrast, the yields were increased for CL bio-crude (from 32.40% to 41.19%), SS hydrochar (from 19.55% to