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Dependence of enzyme on cysteine; serine, and metal ions was confirmed by β-mercaptoethanol; PMSF and EDTA, respectively which induced its partial inhibition. Additionally, protease inhibited in vitro biofilm formation in Staphylococcus aureus. Conclusively, the production of a neutral halo-thermophilic protease is reported for the first time in the genus Halococcus.The formation and use of the scientific names of prokaryotes is governed by the International Code of Nomenclature of Prokaryotes. Originally deriving from the 1935 revision