https://www.selleckchem.com/pr....oducts/smoothened-ag
The immunoblotting technique (also known as Western blotting) is an essential tool used in biomedical research to determine the relative size and abundance of specific proteins, as well as protein modifications. However, long incubation times severely limit its throughput. We have devised a system that improves antigen binding by cyclic draining and replenishing (CDR) of the antibody solution in conjunction with an immunoreaction enhancing agent. Biochemical analyses revealed that the CDR method reduced the incubation
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