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Head-to-tail trimers are designed by intermolecular communications involving the G domain and HB2-LIS. Biochemical as well as in vivo analyses support the indisputable fact that the installation interfaces observed here are native and important for Mgm1 purpose. We additionally discovered that s-Mgm1 interacts with negatively recharged lipids via both the G domain and LIS. Centered on these findings, we suggest that membrane targeting via the G domain and LIS facilitates the in cis installation of Mgm1, possibly p