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To check if the greater amount of theoretically available centrosome marker pericentrin could act as a surrogate for centriole amplification in CTCs, cells were stained with pericentrin and centrin antibodies to guage CA. This assay was very first validated using breast cancer tumors cell outlines and a non-transformed epithelial cell range model of inducible CA, then translated to CTCs. Pericentrin location and pericentrin location x intensity correlate well with centrin foci, v
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