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Using long-acting testosterone undecanoate injections resulted in a more prominent decrease in serum estradiol values over 12 months, compared with short-acting mixed testosterone esters (p  less then  0.001) or testosterone gel (p = 0.001). Changes in serum estradiol were positively correlated to changes in luteinizing hormone (ρ = 0.107, p  less then  0.001) and negatively correlated to changes in follicle-stimulating hormone levels (ρ = -0.167, p  less then  0.001) and body mass index (ρ = -0.082, p  less then  0.001). Conclusion Te