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Double functional polymeric micelles (PMs) have now been emerged as powerful nanocarriers for combinational disease therapy. In our study, the possibility of tri-layer PMs laden with anti-nuclear factor-κB (NF-κ siRNA and 4-(N)-stearoyl gemcitabine (GemC18) has been examined for cancer tumors therapy. PMs with various core hydrophobicity had been served by using poly(ε-caprolactone), polyethyleneimine and polyethylene glycol (PCL-PEI-PEG) copolymers and evaluated. The re