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48%-101.81%) of surrogate analytes, and precision (RSD intra-day, 1.06%- 11.51%; inter-day, 2.23%- 11.38%), accuracy (RE, -7.40%-10.76%) and stability (87.37%-111.70%) of authentic analytes. We successfully applied this method to evaluate the pharmacokinetics of CBBP and NBBP in rats, which revealed critical in vivo properties of both bear bile preparations. This article is protected by copyright. All rights reserved.BACKGROUND Aggressive acceptance of liver allografts has driven utilization of marginal allografts. Our aim was to ass