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Future researchers should focus on including larger and more diverse samples, lengthier longitudinal designs, stronger control conditions, and standardized dosages. The supernatant from rat intestinal acetone powder (RIAP) was used as a source of mammalian glucosidases to determine the digestion properties of glycemic-carbohydrates. We hypothesized that many glucosidases are still anchored to the precipitated-intestinal tissues with available enzymes, and developed a method using the RIAP suspension to optimize the in vitro carbohydra