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©2020 BMFH Press.The facultative anaerobic bacterium Lactobacillus casei IGM394 is used as a number for medicine distribution methods, also it displays the same growth rate under cardiovascular and anaerobic conditions. L. casei strains carry a few genetics that facilitate oxygen and reactive air species (ROS) tolerance within their genomes, however their full functions haven't been uncovered. To simplify the air and ROS tolerance components of L. casei IGM394, we constructed 23